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Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
SH3PXD2A-PX (1-126)
Plasmid#119115PurposeBacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)DepositorInsertSH3PXD2A-PX (1-126) (SH3PXD2A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMay 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SH3(5R)
Plasmid#112154PurposeMammalian expression plasmid for SH3-5R in a vector expressing mCherry.DepositorInsertSH3-5R (NCK1 Human)
UseTagsExpressionMammalianMutationonly the second SH3 domain is expressed in this c…PromoterCMVAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP5
Plasmid#23579DepositorInsertSH3BP5 (SH3BP5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-short-UBASH3A
Plasmid#192088PurposeMammalian expression of human wild-type short-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP5L
Plasmid#23566DepositorInsertSH3BP5L (SH3BP5L Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SH3BP4
Plasmid#23643DepositorInsertSH3BP4 (SH3BP4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-2300
Plasmid#170985PurposeEncodes sgRNA target 2300 and Tetracycline resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-WT-long-UBASH3A
Plasmid#192087PurposeMammalian expression of human wild-type long-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-sh3bgr
Plasmid#192727PurposeGateway entry vector encoding zebrafish sh3bgrDepositorInsertsh3bgr (zgc:100896 Zebrafish)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#2
Plasmid#163401PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#3
Plasmid#163402PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#1
Plasmid#163400PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh3
Plasmid#168985PurposeExpresses RFP cDNA and SAE1 shRNA 3 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-2
Plasmid#160068PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ 3m shRNA-1
Plasmid#160067Purpose3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGGcTGaTTaCAACAAGGATTG (mutations shown in lower c…Promotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-1
Plasmid#160066PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only