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Plasmid#110300PurposeExpresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pENTR_Mm_CEP55(WT)
Plasmid#136333PurposeMouse CEP55 cDNA in Gateway pENTRDepositorInsertCEP55 (Cep55 Mouse)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-PBD H538A/K540M
Plasmid#60879PurposeBacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutantDepositorInsertPolo-like kinase 1 (PLK1 BC01486, Human)
UseTagsGSTExpressionBacterialMutationH538A/K540MPromoterAvailable sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_Hu_ECT2(WT)
Plasmid#136330PurposeLentiviral expression of human ECT2 WTDepositorInsertECT2 (ECT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Mm_MyoGEF(WT)
Plasmid#136325PurposeMammalian expression of mouse MyoGEF-WT with N-terminal EGFPDepositorInsertPLEKHG6 (Plekhg6 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_Hu_ECT2(T327D)
Plasmid#136332PurposeLentiviral expression of human ECT2_T327DDepositorInsertECT2 (ECT2 Human)
UseLentiviralTagsExpressionMammalianMutationT327DPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Mm_MyoGEF(WT)
Plasmid#136327PurposeMouse MyoGEF-WT in Gateway pENTRDepositorInsertPLEKHG6 (Plekhg6 Mouse)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Mm_MyoGEF(T575A)
Plasmid#136328PurposeMouse MyoGEF-T575A mutant in Gateway pENTRDepositorInsertPLEKHG6 (Plekhg6 Mouse)
UseGateway entry vectorTagsExpressionMutationT575APromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP_Hu_p115-RhoGEF(WT)
Plasmid#136337PurposeMammalian expression of human p115-RhoGEF with N-terminal EGFPDepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-V5_Hu_p115-RhoGEF(WT)
Plasmid#136335PurposeMammalian expression of human p115-RhoGEF with N-terminal V5DepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-hMeikin(8A mut)
Plasmid#174707PurposeDoxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 bindingDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationS175A, T176A, T180A, S181A, S196A, T251A, T264A, …PromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA315
Plasmid#215951PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethalDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA310
Plasmid#215950PurposeFragmid fragment: (guide cassette) guide expression; positive control lethalDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Myristoylated Kinase Library
Plasmid Kit#1000000012Purpose190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
CCSB-Broad Human Kinase ORF Collection
Plasmid Kit#1000000014Purpose556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only