-
Plasmid#117646PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0021
Plasmid#117532PurposeLevel0 Golden Gate part: CDS, SaCas9 (no stop codon)DepositorInsertSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0016
Plasmid#117526PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0DepositorInsertSp-eCas9 1.0 (with stop codon)
UseCRISPRTagsExpressionMutationK810A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0011
Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRTagsExpressionMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_reporter_vector
Plasmid#221460PurposeVector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.DepositorInsertgypsy insulator-EGFP-neutral spacer DNA-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_ZF1_mNeonGreen
Plasmid#213834PurposeFacilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_mNeonGreen_ZF2
Plasmid#213835PurposeFacilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_ZF2_mNeonGreen
Plasmid#213836PurposeFacilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_mNeonGreen_ZF1
Plasmid#213837PurposeFacilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_mNeonGreen
Plasmid#213838PurposeFacilitates genomic integration of the control reporter without zinc finger binding sites.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF1_mNeonGreen
Plasmid#213840PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-1DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_ZF2_mNeonGreen
Plasmid#213841PurposeFacilitates genomic integration of the reporter with upstream binding sites for ZF-2DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pReporter_TetR
Plasmid#213847PurposeFacilitates genomic integration of tetR expression cassette.DepositorInsertTetR
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ADIPOR2_FOXJ2
Plasmid#205768PurposeExpress mEGFP-tagged fusion protein, ADIPOR2_FOXJ2 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0068
Plasmid#196179PurposeYFP expression cassette: CaMV35S::TMV::YFP::35sTDepositorInsertCaMV35S::TMV::YFP::35sT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12Fuzzy3MYB
Plasmid#194440PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Portal_ST32
Plasmid#188825PurposePortal protein from phage ST32DepositorInsertPortal
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Portal_Lambda
Plasmid#188828PurposePortal protein from phage LambdaDepositorInsertPortal
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN1
Plasmid#194439PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1DepositorInsertgRNA targeting CEN1
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Portal_ZL19
Plasmid#188824PurposePortal protein from phage ZL19DepositorInsertPortal
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only