Skip to main content
Addgene
Showing: 61 - 80 of 28183 results

  3. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

    Enlarge

  4. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

    Enlarge

  6. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

    Enlarge

  7. iCAP-ROOT

    Plasmid
    #192262
    Purpose
    AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid
    Depositor
    Li Ye
    Article
    Wang et al Nature. 2022 Sep;609(7927):569-574. doi:
    Insert
    Synthetic construct for ROOT VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    p41
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192262-plasmid-map-sequence-id-379257

    Enlarge

  8. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

    Enlarge

  11. EcSTH-LbNOX

    Plasmid
    #211924
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.
    Depositor
    Binghui Li
    Article
    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:
    Insert
    EcSTH-LbNOX
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    211924-plasmid-map-sequence-id-419891

    Enlarge

  13. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

    Enlarge

  18. pM-CBE

    Plasmid
    #196293
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding CBE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    196293-plasmid-map-sequence-id-389996

    Enlarge

  19. pGEX-4T-2/Rabaptin-5:R5BD

    Plasmid
    #168808
    Purpose
    Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members
    Depositor
    Guangpu Li
    Article
    Qi et al Methods Mol Biol. 2015;1298:259-70. doi:
    Insert
    Rab5-binding domain (aa739-862) of Rabaptin-5
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168808-plasmid-map-sequence-id-331194

    Enlarge

Showing: 61 - 80 of 28183 results