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Plasmid#175459PurposeProtein expression in bacterial cells. FERM domain, M1-L390.DepositorInsertEPB41L3 (EPB41L3 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CNN3A-c007
Plasmid#175457PurposeProtein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.DepositorInsertCNN3 (CNN3 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c025
Plasmid#139762PurposeProtein expression/GST pull-downDepositorInsertTBXT, Full-length, G177D (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c024
Plasmid#139761PurposeProtein expression/GST pull-downDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids E41-D225PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c023
Plasmid#139760PurposeProtein expression/GST pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationContains only amino acids E41-D225PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c022
Plasmid#139759PurposeProtein expression/crystallographyDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationG177D; contains only amino acids E41-A214PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c005
Plasmid#139754PurposeFor protein expression/crystallography (PDB:6F58)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6ExpressionBacterialMutationContains only amino acids E41-S224PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
BL21(DE3)-R3-pRARE2
Bacterial Strain#26242PurposePhage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.DepositorBacterial ResistanceChloramphenicolAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionBacterialMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-Y117C-FL
Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-2
Plasmid#175362PurposeG418R backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1
Plasmid#175360PurposeZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-1x
Plasmid#124690PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-2x
Plasmid#124691PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-12-C
Plasmid#124693PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-9-G
Plasmid#124692PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-6-C,16-C
Plasmid#124694PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-miSFIT-10-G, 15-C
Plasmid#124695PurposeTuning expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only