Addgene

We narrowed to 2,350 results for: GCG

Showing: 61 - 80 of 2350 results

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UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

Plasmid

#218860

Purpose

10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCG-ICAM(1235)-nrxn1b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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elavl3:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218852

Purpose

D. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Use

Tags

QF fusion protein

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Use

Tags

QF fusion protein

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194375

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194376

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194377

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

Plasmid

#121175

Purpose

Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

Depositor

Timo Otonkoski

Insert

GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A, R280A,K284A]

Plasmid

#188599

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A]

Plasmid

#188596

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG]

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAWp92

Plasmid

#157975

Purpose

pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone32 (Opti-SpCas9)

Plasmid

#131736

Purpose

Expresses Opti-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

Opti-SpCas9

Use

Lentiviral

Tags

Flag

Expression

Mutation

R661A+K1003H

Promoter

EFS

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp100

Plasmid

#157977

Purpose

pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp40

Plasmid

#157980

Purpose

pFUGW-EFSp-BFP-{SbfI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Expression

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Botch [N116/14R] (Recombinant Antibody)

Antibody

#191818-rAb

Purpose

Anti-Botch (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunocytochemistry

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available since

March 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pAWp28-v1 scaffold

Plasmid

#157976

Purpose

pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAWp102-v2 scaffold

Plasmid

#157978

Purpose

pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Wolfe-Lawson ZFN modular assembly kit

Plasmid Kit

#1000000018

Purpose

Contains reagents for engineering zinc finger arrays by the modular assembly method.

Depositor

Nathan Lawson, Scot Wolfe

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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