Addgene

We narrowed to 6,013 results for: FRI;

Showing: 61 - 80 of 6013 results

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membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpGalpha-i

Plasmid

#185568

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

Galpha-i

Use

In vitro transcription (mrna synthesis)

Tags

GFP, S-Tag

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1-GFP

Plasmid

#185573

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1L-GFP

Plasmid

#185574

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1L

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

K82R kinase-dead mutant

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-AGS-mCherry

Plasmid

#135596

Purpose

Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1

Plasmid

#185571

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1 (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpGalpha-i

Plasmid

#185568

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

Galpha-i

Use

In vitro transcription (mrna synthesis)

Tags

GFP, S-Tag

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-HsPLK1-dead

Plasmid

#185572

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1-dead (PLK1 Human)

Use

In vitro transcription (mrna synthesis)

Tags

membrane targeting sequence from Neuromodulin (ML…

Expression

Mutation

K82R kinase-dead mutant

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1(118-1274)

Plasmid

#221207

Purpose

ScChd1-aa118-1274 chromatin remodeler

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[R126A/R130A]

Plasmid

#221197

Purpose

ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

R126A, R130A

Promoter

T7

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[Y137A]

Plasmid

#221198

Purpose

ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

Y137A

Promoter

T7

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-XlH3[ChEx]

Plasmid

#221200

Purpose

Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3[ChEx]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-Xl-H3∆Nterm[R42]

Plasmid

#221201

Purpose

Xenopus laevis histone H3 with N-terminal tail deleted.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3∆Nterm[R42]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Use

Tags

V5

Expression

Mammalian

Mutation

P101S

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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Klotho (secreted)

Plasmid

#17713

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pGEM-601[insert(+1) TA-rich]

Plasmid

#221196

Purpose

601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

601[insert(+1)TA-rich]

Use

Unspecified

Tags

Expression

Mutation

601 has an additional mutation added 22 bp from d…

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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