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Plasmid#184836PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-amp
Plasmid#184843PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1
Plasmid#184566PurposeInducible expression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS540-pCSDest2-AcrIIC4Hpa
Plasmid#113434PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS542-pCSDest2-AcrIIC5Smu
Plasmid#113435PurposeExpresses type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS
Plasmid#113436PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInserttype II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS
Plasmid#113437PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA4
Plasmid#101043PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA4
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA2
Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS663-pMCSG7-AcrIIC4Hpa
Plasmid#113440PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS664-pMCSG7-AcrIIC5Smu
Plasmid#113441PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES
Plasmid#113438PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES
Plasmid#113439PurposeExpresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X
Plasmid#128112PurposeAcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9DepositorInsertAcrX (AcrIIC1 N3F/D15Q/A48I)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1-mCherry chimera #10
Plasmid#128113PurposeExpresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9DepositorInsertAcrIIC1-mCherry chimera #10
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9(WT)
Plasmid#101044PurposeExpress Streptococcus pyogenes Cas9DepositorInsertSpyCas9
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG
Plasmid#136513PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cellsDepositorInsertcodon-optimized AcrIIA5
UseTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-SpyCas9
Plasmid#113033PurposeSpyCas9 expression in mammalian cells (CMV promoter driven)DepositorInsertSpyCas9
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR
Plasmid#136514PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vectorDepositorInsertCodon-optimized AcrIIA5
UseLentiviralTagsFLAG/NLSExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-NT
Plasmid#184568PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only