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We narrowed to 754 results for: Anti-CRISPR

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Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124283

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124285

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA

Plasmid

#192159

Purpose

AAV-CRISPRa base vector

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1-cloDF-kan

Plasmid

#184836

Purpose

Expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1-cloDF-amp

Plasmid

#184843

Purpose

Expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1

Plasmid

#184566

Purpose

Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS540-pCSDest2-AcrIIC4Hpa

Plasmid

#113434

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS542-pCSDest2-AcrIIC5Smu

Plasmid

#113435

Purpose

Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

Plasmid

#113436

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

Plasmid

#113437

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS663-pMCSG7-AcrIIC4Hpa

Plasmid

#113440

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

lacI

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS664-pMCSG7-AcrIIC5Smu

Plasmid

#113441

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

lacI

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES

Plasmid

#113438

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Inserts

Type II-C anti-CRISPR
mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES

Plasmid

#113439

Purpose

Expresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Inserts

Type II-C anti-CRISPR
mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIC1-mCherry chimera #10

Plasmid

#128113

Purpose

Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrIIC1-mCherry chimera #10

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCas9(WT)

Plasmid

#101044

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

SpyCas9

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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