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Plasmid#100033PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.DepositorInsertRhoGC E254D
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationE254DPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Dhr1
Plasmid#100003PurposeExpress yeast Dhr1 with Y2H activation domain fusionDepositorInsertDhr1 (ECM16 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Dhr1
Plasmid#100002PurposeExpress yeast Dhr1 with Y2H binding domain fusionDepositorInsertDhr1 (ECM16 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Sgd1
Plasmid#100000PurposeShuttle Sgd1 into Gateway destination vectorDepositorInsertSgd1 (SGD1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Bms1
Plasmid#100001PurposeShuttle Bms1 into Gateway destination vectorDepositorInsertBms1 (BMS1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380E
Plasmid#100035PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.DepositorInsertRhoGC D380E
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationD380EPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380N
Plasmid#100034PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.DepositorInsertRhoGC D380N
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationD380NPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
EC1000
Bacterial Strain#71852PurposeRepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmidsDepositorBacterial ResistanceKanamycinAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE
Plasmid#67292PurposeInduce human iN conversion from fibroblastDepositorInsertMyt1L (Myt1l Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFW1000
Plasmid#224210PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW100003
Plasmid#222442PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.DepositorInsertmCherry2-HyP5SM
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW100002
Plasmid#222440PurposeLevel 0, CDS module (AATG-GCTT) containing rice OsL5.DepositorInsertOsL5 (Oryza sativa)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPC1000
Plasmid#174829PurposeLentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit siteDepositorInsertsCRISPRi SpCas9 sgRNA cassette
PuroR-P2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationDetailed in manuscriptPromoterEF1a and mouse U6Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM1000
Plasmid#8840DepositorTypeEmpty backboneUseTagsT. maritima MBPExpressionBacterialMutationPromoterAvailable sinceNov. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1000
Plasmid#44475DepositorInsertMinimal Mos1, PuroR, peel-1, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF1000
Plasmid#142251PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF302 (ZNF302 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-1000 to -1)
Plasmid#66128PurposeLuciferase-based reporter for VEGF promoter region (-1000 to -1 bp)DepositorInsertVascular Endothelial Growth Factor -A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -1000 to -1Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Brn2.WPRE
Plasmid#67294PurposeInduce human iN conversion from fibroblastDepositorInsertBrn2 (Pou3f2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE
Plasmid#67291PurposeInduce human iN conversion from fibroblastDepositorInsertAsc1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE
Plasmid#67293PurposeInduce human iN conversion from fibroblastDepositorInsertMyt1L (Myt1l Mouse)
UseLentiviralTagsExpressionMammalian and YeastMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only