Addgene

We narrowed to 24,648 results for: cat

Showing: 7961 - 7980 of 24648 results

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pLKO.1-scrCdh20.1 GFP

Plasmid

#209106

Purpose

GFP expressing scramble of shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh20.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pU3-gRNA

Plasmid

#53063

Purpose

Transcription of guide RNA (gRNA) in rice cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

OsU3 promoter + gRNA scaffold

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

rice U3

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-GGPPipi

Plasmid

#53280

Purpose

Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-3HA

Plasmid

#41609

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, TRP1 reverse orientation, and yeast ADH1 te…

Expression

Mutation

Promoter

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

Plasmid

#50022

Purpose

expresses Membrane tagged G-CaMP3 in cells expressing Cre

Depositor

David Anderson

Article

Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

Insert

G-CaMP3

Use

AAV

Tags

MARCKS sequence (MGCCFSKT)

Expression

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP1-Halo

Plasmid

#175300

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

Plasmid

#135460

Purpose

Mammalian expression of uFLARE TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape

Use

Tags

HA, flag,

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2 K52R

Plasmid

#118298

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 K52R (Mapk1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

K52R

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

myo-2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle-HA-FLAG-TIN2

Plasmid

#35025

Depositor

Sheila Stewart

Article

Nittis et al Mol Cell Proteomics. 2010 Jun;9(6):1144-

Insert

TIN2 (TINF2 Human)

Use

Adenoviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
BcLOV-mCh-SOS

Plasmid

#208279

Purpose

Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCh-SOS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pECFP

Plasmid

#70106

Purpose

mammalian expression of hSERT with C-terminal CFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pU6-gRNA

Plasmid

#53062

Purpose

Transcription of guide RNA (gRNA) in wheat cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

wheat U6 promoter - gRNA

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

wheat U6

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM2-N-22

Plasmid

#54164

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM2

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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LbCas12a

Plasmid

#188493

Purpose

Expresses FLAG tagged LbCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA4 (MSW#517)

Plasmid

#208070

Purpose

Expression of GFP-tagged hsRPA4 in human cells

Depositor

Marc Wold

Article

Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

Insert

RPA4 (RPA4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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OPN(-213)-luc

Plasmid

#31107

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

promoter region -213 to +87

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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