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Plasmid#103119PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG418R-MCS-QF-MiniWhite
Plasmid#165902PurposeG418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc
Plasmid#138367PurposeLentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A0LWB3
Plasmid#103070PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
EZH2-TALEN_Right_No_FLAG
Plasmid#79900PurposeUntagged EZH2-TALEN_Right to target the N-terminus of human EZH2DepositorInsertEZH2-TALEN_Right_No_FLAG (EZH2 Human)
UseTALENTagsExpressionMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(498-663)
Plasmid#35070DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 498-663PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
B9L-BirA*
Plasmid#109146PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertB9L (Bcl9l Mouse)
UseTagsBirA(R118G) and FlagExpressionMammalianMutationPromoterCMV/TetO2Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAav-MCS-PQS1-3xFLAG
Plasmid#84883PurposerAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertMulticloning sites and selection cassette
UseAAVTagsExpressionMutationPromoterPGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6I4Q5
Plasmid#103101PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1316-1538)
Plasmid#35079DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 1316-1538PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(540-758)
Plasmid#35071DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionBacterialMutationContains aa 540-758PromoterT7Available sinceMay 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLS2965
Plasmid#122534PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_aman-2::CemOrange2 (aman-2 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_Lys322Arg
Plasmid#105862PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationLys322Arg in constant region of IgG1 heavy chainPromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeQ-GFP]
Plasmid#153540PurposeAAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeQ-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Fn
Plasmid#138104PurposeGolden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Fn
Plasmid#138095PurposeGolden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-Fn
Plasmid#138098PurposeGolden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-Fn
Plasmid#138101PurposeGolden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC148-pCR8-dCas9VP96
Plasmid#48220PurposedCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP96ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only