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Plasmid#223625PurposeLentiviral expression of human CD28 (Y191F) in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsExpressionMutationY191FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28
Plasmid#223622PurposeLentiviral expression of human CD28 with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28-LgBiT
Plasmid#223616PurposeLentiviral expression of human CD28 with LgBiT tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsLgBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28-CLIP
Plasmid#223610PurposeLentiviral expression of human CD28 with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28
Plasmid#223600PurposeLentiviral expression of human CD28 with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-CERT(PHD)
Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)(W33A)
Plasmid#221761PurposeStable expression of GFP-CERT(PHD)(W33A) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116, mutated 33W to 33APromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-Btk1(PHD)-GFP
Plasmid#221756PurposeStable expression of Btk1(PHD)-GFP in mammalian cellsDepositorInsertBtk (BTK Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–177PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TRPML1(PHD)
Plasmid#221752PurposeStable expression of GFP-TRPML1(PHD) in mammalian cellsDepositorInsertTRPML1 (MCOLN1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TRPML1(PHD) (amino acids 1–69)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-ING2(PHD)
Plasmid#221751PurposeStable expression of GFP-ING2(PHD) in mammalian cellsDepositorInsertING2 (ING2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtwo ING2(PlantHD) domains (amino acids 190–280)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)
Plasmid#221746PurposeStable expression of GFP-CERT(PHD)in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_SAT1 (human)
Plasmid#220477Purposeheterologous expression of SSAT1 under endogenous speG E. coli promoterDepositorInsertSSAT1 (SAT1 Human)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
95.b_pSTC0-zeo
Plasmid#202335PurposeDesign 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert95.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
90.b_pSTC0-zeo
Plasmid#202337PurposeDesign 90.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert90.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
80.b_pSTC0-zeo
Plasmid#202339PurposeDesign 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert80.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
70.b_pSTC0-zeo
Plasmid#202341PurposeDesign 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert70.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
50.b_pSTC0-zeo
Plasmid#202343PurposeDesign 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert50.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.a_pSTC0-zeo
Plasmid#202347PurposeDesign opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.b_pSTC0-zeo
Plasmid#202348PurposeDesign opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
rw-consensus_pSTC0-zeo
Plasmid#202349PurposeControl rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertrw-consensus
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only