Addgene

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pRAJ31f

Plasmid

#39257

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no terminator for the transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pRAJ11m

Plasmid

#39245

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pAG500-FKBP-N-frFAST

Plasmid

#160060

Purpose

Expresses FKBP-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FKBP-N-frFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL088

Plasmid

#137896

Purpose

iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL082

Plasmid

#137891

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP2

Plasmid

#66582

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pRVV584

Plasmid

#108280

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRAJ11

Plasmid

#39244

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12m

Plasmid

#39247

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21m

Plasmid

#39250

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Tags

Expression

Worm

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHdCas9-mammalian

Plasmid

#138415

Purpose

Expression of Himar-dCas9 in mammalian cells

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Use

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGGD_RT_li_Venus

Plasmid

#136973

Purpose

Goldengate/Greengate C-tag module, containing Venus fused to a flexible linker

Depositor

Klaus Theres

Article

Lopez et al Plant Cell. 2021 Aug 13;33(7):2412-2430.

Insert

Venus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX Shark-tSH2

Plasmid

#126672

Purpose

Expression of Shark tandem SH2 domains SNAP tagged for use as FRET reporter

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark tandem SH2 domains

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFLLFLN

Plasmid

#40030

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-Lox5171-Lox2272-FRT-loxP
Neo

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

None and SV40

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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