Addgene

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SH2-B-SH2 RA

Plasmid

#46480

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B RK (SH2B1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639; R555A mutation in t…

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-876-3p

Plasmid

#103742

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-876-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-876-3p target (MIR876 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-377-3p

Plasmid

#103501

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-377-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-377-3p target (MIR376C Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-34a-5p

Plasmid

#103465

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-34a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-34a-5p target (MIR34A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-377-5p

Plasmid

#103502

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-377-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-377-5p target (MIR377 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-203a-3p

Plasmid

#103336

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-203a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-203a-3p target (MIR203A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-301a-3p

Plasmid

#103396

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-301a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-301a-3p target (MIR301A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-505-3p

Plasmid

#103583

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-505-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-505-3p target (MIR505 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-1307-3p

Plasmid

#103214

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-1307-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-1307-3p target (MIR1307 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-30c-1-3p

Plasmid

#103413

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-30c-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-30c-1-3p target (MIR30C1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-27a-3p

Plasmid

#103381

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-27a-3p target (MIR27A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-17-3p

Plasmid

#103273

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-17-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-17-3p target (MIR17 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-169

Plasmid

#31052

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -16…

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-136

Plasmid

#31051

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -13…

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-23a-5p

Plasmid

#103372

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-23a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-23a-5p target (MIR23A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

Plasmid

#58414

Purpose

Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA internal

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine; The sequenc…

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
LSB-hsa-miR-25-5p

Plasmid

#103376

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-25-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-25-5p target (MIR25 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7e-5p

Plasmid

#103153

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7e-5p target (MIRLET7E Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7e-3p

Plasmid

#103152

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7e-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7e-3p target (MIRLET7E Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge