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Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK4-P2A-Hygro_Barcode
Plasmid#170223PurposeBarcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mTagBFP2-Blasticidin
Plasmid#167207PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with BlasticidinDepositorTypeEmpty backboneUseCRISPRTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)
Plasmid#214880PurposeLentiviral vector encoding RfxCas13d targeting CD46 guideDepositorInserthU6-crCD46-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP-Bcr in pCCL-cppt178-MNDU3
Plasmid#38156DepositorInsertBcr (BCR Human)
UseLentiviralTagsECFPExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Xph15-GCaMP6f
Plasmid#135538PurposeEncodes a specific PSD-95 binder (Xph15) fused to GCaMP6fDepositorInsertXph15
UseTagsGCaMP6fExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2-Kv2.1PR
Plasmid#195522PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJC-repo1.9kb-TALE4-VP64-P10
Plasmid#104612PurposeExpresses TALE4 under the control of a 1.9kb repo enhancer elementDepositorInsert1.9kb repo enhancer element (repo Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP(iL)ExpressionYeastMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-pAceR-Kv2.1PR
Plasmid#195531PurposeRed fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)
Plasmid#214877PurposeLentiviral vector encoding RfxCas13d targeting LPT guide arrayDepositorInserthU6-crLPT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
Plasmid#192160PurposeDual AAV vector AAV-dCas9DepositorInsertDead Cas9
UseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB3-miRFP670nano
Plasmid#127433PurposeEB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertEB3-miRFP670nano (MAPRE3 Synthetic, Human)
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA
Plasmid#104563PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)DepositorInsertdLbCpf1 (D832A)
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
UseTagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD3
Plasmid#173851PurposeProtein expression plasmid for recombinant His-Myc tag human PLD3DepositorInsertPLD3 (PLD3 Human)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP160ExpressionMutationD10A;H840A nuclease-deficientPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-EGFP-T2A-Bsd
Plasmid#193198PurposeLentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance markerDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KIF1A(1-393)-GCN4-2xmCherry-EF(C)
Plasmid#61665PurposeTandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linkerDepositorInsertKIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only