Addgene

We narrowed to 18,521 results for: omp

Showing: 7821 - 7840 of 18521 results

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pSD118A

Plasmid

#218140

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0_ZF2-3

Use

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD068F

Plasmid

#218137

Purpose

Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36-eGFP-IRES2-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD057C

Plasmid

#218136

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD113A

Plasmid

#218133

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM189A

Plasmid

#218135

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM195A

Plasmid

#218125

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
M13 pIII

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM194A

Plasmid

#218124

Purpose

Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN_CTD
luxAB

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO CRYGS 16-177 Q16C

Plasmid

#217673

Purpose

N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

Depositor

Larry David

Insert

CRYGS (CRYGS Human)

Use

Tags

6XHis and SUMO

Expression

Bacterial

Mutation

Glutamine 16 changed to Cysteine, and amino acids…

Promoter

T7

Available since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMV1 pEF1a-HGF

Plasmid

#188748

Purpose

Vector constitutively expressing HGF

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

HGF (HGF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

Plasmid

#195543

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Plasmid

#202064

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Plasmid

#202059

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator

Plasmid

#202061

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Insert

AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITRs intact

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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gWiz_BM40-vGAT-VL-hIgG1-CL

Plasmid

#218437

Purpose

mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

Depositor

Axel Brunger

Article

Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

Insert

vGAT-VL-hIgG1-CL

Use

Affinity Reagent/ Antibody

Tags

BM40

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-mApple-6xHp

Plasmid

#217376

Purpose

Lipid droplet localization of 6xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

mApple-6x Hp NC

Use

Lentiviral

Tags

mApple

Expression

Mutation

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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RC(14-3-3ε)-LgBit

Plasmid

#208926

Purpose

Recognition domain for in vitro RSK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

14-3-3ε (YWHAQ Human)

Use

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(ER-mito)

Plasmid

#214427

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Use

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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Drosophila Wdr20-V5

Plasmid

#217622

Purpose

Mammalian expression of V5 tagged Drosophila Wdr20

Depositor

Ethan Lee

Article

Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

Insert

WDR20

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-ER

Plasmid

#214413

Purpose

ER localization of CFAST fused to Halotag-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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