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Addgene
Showing: 7821 - 7840 of 20568 results
  1. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pZNF639-donor

    Plasmid
    #112345
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF639 with GFP
    Depositor
    Insert
    ZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:179333542:G>T,rs978050614
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pUSF2-donor

    Plasmid
    #112361
    Purpose
    CRISPR donor plasmid to tag human transcription factor USF2 with GFP
    Depositor
    Insert
    USF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:35278400:T>A, hg38:19:35278455:G>C
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pZNF140-donor

    Plasmid
    #112363
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF140 with GFP
    Depositor
    Insert
    ZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:133105834:A>G, hg38:12:133105852:A>G
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pZNF250-donor

    Plasmid
    #112383
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF250 with GFP
    Depositor
    Insert
    ZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:144882271:C>G,hg38:8:144882273:T>C
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pNR2F1-donor

    Plasmid
    #112388
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR2F1 with GFP
    Depositor
    Insert
    NR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs974916569
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs4864608
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  10. Cas9-T2A-H2B mCitirine

    Plasmid
    #210471
    Purpose
    Coexpression of Cas9 and H2B mCitrine
    Depositor
    Insert
    Cas9-T2A-H2B mCitirine
    Use
    CRISPR and Synthetic Biology
    Tags
    mCitrine on C terminal of H2B
    Expression
    Mammalian
    Mutation
    Cas9 contains 3xFLAG tag and nuclear localization…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Availability
    Academic Institutions and Nonprofits only
Showing: 7821 - 7840 of 20568 results