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Plasmid#67537PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only
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pJexpress414_OmpA171_R4cons_Y129H
Plasmid#64302PurposeConservative version of OmpA Redesign 4 with Y129H mutationDepositorInsertOmpA_R4cons_Y129H
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutation25 mutations to lipid-facing surfacePromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-MET17
Plasmid#122885PurposeADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-LEU2
Plasmid#122888PurposeLYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-HIS3
Plasmid#122891PurposeTRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-MET17
Plasmid#122893PurposeTRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHAGE-E-cadherin-RFP
Plasmid#79603PurposepHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging systemDepositorInsertCDH1 promoter region (CDH1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
Plasmid#31483DepositorInsertTraffic Light Reporter 2.1 (V2468 zfn target) EF Puro
UseLentiviralTagsExpressionMutationTraffic Light 2.0 series have a +3 PEST sequence …PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-EGFP
Plasmid#63592PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.DepositorInsertsCas9
EGFP
UseCRISPR and LentiviralTagsFLAG and P2AExpressionMammalianMutationPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cx30-msfGFP
Plasmid#69019PurposeExpresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx30 (GJB6 Human)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromotercAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-DENV3-NS5FL
Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
UseTagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-CFP;UBC-Cre25nt
Plasmid#65727PurposeExpresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.DepositorInsertsCMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression
UseCre/LoxTagsExpressionMammalianMutationPromoterCMV and UBCAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-NLS-TPP1
Plasmid#53585PurposeMammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.DepositorInsertTPP1 (ACD Human)
UseTags3xFLAG and NLSExpressionMammalianMutationSilent mutation to eliminate PacI sitePromoterCMVAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFR L858R
Plasmid#11012PurposeRetroviral construct for expressing human EGFR with L858R mutation in human cellsDepositorInsertEGFR L858R (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R (tumor-derived)PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
Plxnb2(S)-Fc-His
Plasmid#72129PurposeExpresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.3-AP-His
Plasmid#71974PurposeExpresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNrp2.3 (Nrp2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5B-FLAG
Plasmid#72195PurposeExpresses full-length Unc5b with a C-terminal FLAG tagDepositorInsertUnc5b (Unc5b Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only