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Plasmid#176215PurposeExpresses GFP under the control of distal enhancer and the promoter of human adiponectin geneDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-Lepr_FL-IRES-eGFP
Plasmid#196635PurposeDoxycycline inducible expression of full length mouse Lepr with eGFP markerDepositorInsertLepr (Lepr Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACE2
Plasmid#164219PurposeExpresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection markerDepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationsilent mutation to remove internal EcoRI site (GA…PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral mBmal1-Luc
Plasmid#182762PurposeLentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporterDepositorInsertmouse arntl promoter (Bmal1 Mouse)
UseLentiviralTagsExpressionMutationPromoter649 bpAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nxph4-2A-CreERT2 Targeting Vector
Plasmid#62219PurposeTargets CreERT2 to the mouse Nxph4 stop codonDepositorInsertNxph4-2A-CreERT2 (Nxph4 Mouse, Bacteriophage)
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterMouse Nxph4Available sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-lamin A
Plasmid#175107PurposeLentiviral expression of V5-tagged mouse LmnaDepositorInsertLmna (Lmna Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-BetaActin-SecA5-YFP
Plasmid#105664PurposeUbiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporterDepositorInsertSecreted Annexin5-EYFP (ANXA5 Human)
UseTol2-gatewayTagsEYFPExpressionMutationPromoterBeta-ActinAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA
Plasmid#177576PurposeExpresses SOUL in neuronal cell typesDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterhSynAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABE7.10-F148A
Plasmid#132946PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertABE7.10(F148A)
UseCRISPR; Base editorTagsExpressionMammalianMutationABE7.10(F148A)PromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6
Plasmid#192184PurposeClone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6DepositorInsertClone 6 heavy chain (HH06)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2
Plasmid#192183PurposeClone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2DepositorInsertClone 2 heavy chain (HH02)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIV-INK4A-zsGreen
Plasmid#110728PurposeMammalian Expression of p16INK4A IRES zsGreenDepositorInsertp16INK4A IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-AktDN
Plasmid#53597Purposelentiviral expression of dominant negative mouse Akt1 and EGFPDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationDominant negative (K179M)PromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
UseTagsExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA
Plasmid#177577PurposeExpresses SOUL in Cre-positive cellsDepositorInsertSOUL
UseAAVTagstdTomatoExpressionMutationPromoterEF1aAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
UseTags6xHis tag on N-terminusExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPbcas13b
Plasmid#89906PurposeBacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.DepositorInsertCas13b
UseTagsExpressionBacterialMutationPromoterLacAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-SaCas9-NLS-VPR
Plasmid#68496PurposeAAV vector containing SaCas9 fused to VPRDepositorInsertSaCas9-VPR
UseAAV and CRISPRTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only