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Addgene
Showing: 7801 - 7820 of 20568 results

  1. pZBTB17-donor

    Plasmid
    #112387
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB17 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB17 homology arms flanking EGFP-IRES-Neo cassette (ZBTB17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112387-plasmid-map-sequence-id-229851

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  2. pZBTB12-donor

    Plasmid
    #113802
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB12 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB12 homology arms flanking EGFP-IRES-Neo cassette (ZBTB12 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs9267664, rs9267663
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113802-plasmid-map-sequence-id-230652

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  3. pZNF696-donor

    Plasmid
    #113806
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF696 with GFP
    Depositor
    Kevin White
    Insert
    ZNF696 homology arms flanking EGFP-IRES-Neo cassette (ZNF696 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs7386259, rs3750217, rs7387702
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113806-plasmid-map-sequence-id-231036

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  4. pTCFL5-donor

    Plasmid
    #113808
    Purpose
    CRISPR donor plasmid to tag human transcription factor TCFL5 with GFP
    Depositor
    Kevin White
    Insert
    TCFL5 homology arms flanking EGFP-IRES-Neo cassette (TCFL5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:20:62842215:T>A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113808-plasmid-map-sequence-id-229886

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  5. pTSHZ2-donor

    Plasmid
    #113810
    Purpose
    CRISPR donor plasmid to tag human transcription factor TSHZ2 with GFP
    Depositor
    Kevin White
    Insert
    TSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113810-plasmid-map-sequence-id-229895

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  6. pTOX2-donor

    Plasmid
    #113819
    Purpose
    CRISPR donor plasmid to tag human transcription factor TOX2 with GFP
    Depositor
    Kevin White
    Insert
    TOX2 homology arms flanking EGFP-IRES-Neo cassette (TOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113819-plasmid-map-sequence-id-230186

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  7. pZNF423-donor

    Plasmid
    #113822
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF423 with GFP
    Depositor
    Kevin White
    Insert
    ZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113822-plasmid-map-sequence-id-230193

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  8. pTFAP2B-donor

    Plasmid
    #113823
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFAP2B with GFP
    Depositor
    Kevin White
    Insert
    TFAP2B homology arms flanking EGFP-IRES-Neo cassette (TFAP2B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Unknown
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113823-plasmid-map-sequence-id-230195

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  9. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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  10. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  12. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldoš
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  13. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  14. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  17. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  20. pTBPL1-donor

    Plasmid
    #112316
    Purpose
    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP
    Depositor
    Kevin White
    Insert
    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs35776966, hg38:6:133987646:GTGTAT>-
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112316-plasmid-map-sequence-id-228480

    Enlarge

Showing: 7801 - 7820 of 20568 results