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pHR-PGK-eGFP-P2A-CRY2-VPR

Plasmid

#192610

Purpose

Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

eGFP-P2A-CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Tags

eGFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.71

Plasmid

#184983

Purpose

Express -Eco1 RT/Cas9 fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker1-Cas9

Use

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-9S-S2 [rpoA]

Plasmid

#176217

Purpose

Expression of TRX-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

HIS and TRX

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-9P-S2 [rpoA]

Plasmid

#176218

Purpose

Expression of TRX-9P-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9P-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-9S-DYW [rpoA]

Plasmid

#176220

Purpose

Expression of TRX-9S-DYW [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-DYW [rpoA]

Use

Synthetic Biology

Tags

TRX-His

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-avi-9S-S2 [rpoA]

Plasmid

#176223

Purpose

Expression of TRX-AviTag-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-Avi-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His-AviTag

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYI047

Plasmid

#170843

Purpose

(pFRO6::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pFRO6::SMT2-FLAG

Use

Tags

Expression

Plant

Mutation

Promoter

FRO6p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI048

Plasmid

#170844

Purpose

(pGH3.17::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pGH3.17::SMT2-FLAG

Use

Tags

Expression

Plant

Mutation

Promoter

GH3.17p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI012

Plasmid

#170841

Purpose

pGH3.17::GUSPlus::tOCS

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GH3.17p

Use

Tags

Expression

Plant

Mutation

Promoter

GH3.17p

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI046

Plasmid

#170842

Purpose

(pUBQ10::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pUBQ10::SMT2-FLAG::tOCS

Use

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_3EGFP

Plasmid

#185967

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-Egfp cassette in forward direction in Network Brick

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PR

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-tdTomato-WPRE

Plasmid

#187112

Purpose

FLP-dependent expression of tdTomato under EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJAK175

Plasmid

#178601

Purpose

pAP259 derived plasmid encoding a xylose inducible BitlucOpt

Depositor

Robert Fagan

Article

Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

Insert

Bitlucopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pxyl

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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