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pCAMTA2.1.0-gDNA
Plasmid#132456PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCAMTA2 (CAMTA2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBD1.1.0-gDNA
Plasmid#132444PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertMBD1 (MBD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
6NIM
Plasmid#127294PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertPP-BRD18
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJune 26, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
5VS7 (PP-BRD4)
Plasmid#101264PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD4
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
5KO4 (PP-BRD19)
Plasmid#101882PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD19
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
5TCK (PP-BRD3)
Plasmid#101887PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
5U9N (PP-BRD3)
Plasmid#101255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
4QNY
Plasmid#61958PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLdBPK_331470.1:MAC04E-A10:C228347
UseTagsMHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-MAPK2 (3N9X)
Plasmid#28117PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPBG-PF11_0147
UseTagsHis tag with TEV siteExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK3
Plasmid#28151PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCDPK3
UseTagsHis tag with TEV cleavageExpressionBacterialMutationCAD domain (residues 425-602).PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
CROPseq-tRFP
Plasmid#230938PurposeModified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPExpressionMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti:FlipGFP-T2A-mCherry
Plasmid#223198PurposeLentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)DepositorInsertFlipGFP-T2A-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-mCherry-attB53
Plasmid#183611PurposeRMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertTRE-mCherry
UseRmceTagsExpressionMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)-Puro
Plasmid#117686PurposeThe plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.DepositorInserteSpCas9(1.1)
UseCRISPRTagsPuroR (R166H)ExpressionMammalianMutationK848A, K1003A, R1060APromoterU6Available sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
OmEF1aCas9P2ApuroSB
Plasmid#165484PurposeStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cellsDepositorInsertZebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
UseCRISPR; TransposonTagsExpressionMutationPromoterOreochromis mossambicus EF1 alpha promoter (OmEF1…Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110561PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertstRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only
