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Plasmid#44092DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only
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pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX601-mCherry
Plasmid#84039PurposeStaphylococcus aureus (SaCas9) conjugated with mCherryDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorInsertMLL-AF4 fusion gene (AFF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterTREAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Tet-on Advanced -BGH-PA
Plasmid#114379PurposeExpress reverse tetracycline inducible rtTA transcriptional activatorDepositorInsertrtTA and BGH-pA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
UseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM Kan + p15A BsaIR Amp
Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p2A-CreERT2-FRT-kan-FRT
Plasmid#108454PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.DepositorInsertmCherry-p2a-CreERT2
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ET_rhi145
Plasmid#180427PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ET_rhi145
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-dsRed
Plasmid#74152PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRedDepositorInsertTol2-kanaR-cryaa-dsRed
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ETh_rhi483
Plasmid#180429PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ETh_rhi483
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-sfGFP
Plasmid#74153PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFPDepositorInsertTol2-kanaR-cryaa-sfGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)
Plasmid#44095DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
Plasmid#44094DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER
Plasmid#52687PurposeSame as 41025, with additional linkerDepositorInserttetO7-Pcyc1-GFP+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)
Plasmid#44089DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only