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Plasmid#76023Purpose3rd generation lentiviral gRNA plasmid targeting human LATS1DepositorInsertLATS1 (LATS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-T1404A
Plasmid#116615PurposeLentiviral expression of PTCH1 T1404ADepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationT1404APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
DTYMK gRNA (BRDN0001147404)
Plasmid#77511Purpose3rd generation lentiviral gRNA plasmid targeting human DTYMKDepositorInsertDTYMK (DTYMK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Flag-FBW7K404R
Plasmid#200717PurposeThe plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
UseTagsFlagExpressionMammalianMutationFBW7 K404 is mutated to Alanine.PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCVbsd-PPARD-A404K
Plasmid#130759PurposeVector encoding PPARD cDNA for retroviral transductionDepositorInsertPPARD A404K (PPARD Human)
UseRetroviralTagsExpressionMutationA404KPromoterAvailable sinceAug. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-A404W
Plasmid#130760PurposeVector encoding PPARD cDNA for retroviral transductionDepositorInsertPPARD A404W (PPARD Human)
UseRetroviralTagsExpressionMutationA404WPromoterAvailable sinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-BRAF-A404Cfs*9
Plasmid#116129PurposeLentiviral expression of BRAF A404Cfs*9DepositorInsertBRAF (BRAF Human)
UseLentiviralTagsExpressionMutationA404Cfs*9PromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAN3 gRNA (BRDN0001146404)
Plasmid#77423Purpose3rd generation lentiviral gRNA plasmid targeting human PAN3DepositorInsertPAN3 (PAN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACVR1C gRNA (BRDN0001149404)
Plasmid#76523Purpose3rd generation lentiviral gRNA plasmid targeting human ACVR1CDepositorInsertACVR1C (ACVR1C Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a404-479.wt
Plasmid#13529DepositorInsertB. subtilis DEAD-box helicase YxiN specific RNA binding domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdelete amino acid residues 1-403PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p404-MTS-mCherry-GFP1-10
Plasmid#91958PurposeExpresses mCherry-GFP1-10 in yeast cell mitochondriaDepositorInsertMTS-mCherry-GFP1-10
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-DuDre-Atg8-404
Plasmid#69201PurposeRFP-Atg8 (PAS marker) with improved propertyDepositorInsertDuDre-Atg8
UseTagsDsRed Express 2ExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2-F404AF407AF409A_V
Plasmid#147813PurposeMammalian Expression of HsDCP2-F404AF407AF409ADepositorInsertHsDCP2-F404AF407AF409A (DCP2 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2404-Tier1-PhCMV-NeoR
Plasmid#169496PurposeTier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).DepositorInsertPCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
JD404: pMVP (L3-L2) BioID2 + WPRE
Plasmid#121797PurposepMVP L3-L2 entry plasmid, contains BioID2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest in lentivirus vectors.DepositorInsertBioID2 + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HL 3722 (=HL 716 + pHL 404)
Plasmid#30084DepositorInsertpLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro HA-hUSP27X S404N
Plasmid#225724PurposeTransfection of USP27X (S404N) with HA tag in mammalian cellsDepositorInsertUSP27X S404N (USP27X Human)
UseTagsHAExpressionMammalianMutationS404NPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 GST-hUSP27X S404N
Plasmid#225717PurposeBacterial expression of USP27X (S404N) with GST tagDepositorInsertUSP27X S404N (USP27X Human)
UseTagsGSTExpressionBacterialMutationS404NPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only