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Plasmid#103661PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-579-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-579-5p target (MIR579 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_W
Plasmid#147902PurposeMammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1resDepositorInsertHsNot9-H58AF60AA64YV71Y-shRNA1res (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_W
Plasmid#147907PurposeMammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1resDepositorInsertHsNot9-Y203AR205AH208AR244A-shRNA1res (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_W
Plasmid#147903PurposeMammalian Expression of HsNot9-R205AH208A-shRNA1resDepositorInsertHsNot9-R205AH208A-shRNA1res (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_W
Plasmid#147908PurposeMammalian Expression of HsNot9-Y203AR244A-shRNA1resDepositorInsertHsNot9-Y203AR244A-shRNA1res (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 HA-TSPAN12^11-LEL
Plasmid#115785PurposeExpresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequenceDepositorUseTagsHAExpressionMammalianMutationlarge extracellular loop of TSPAN12 replaced with…PromoterCMVAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_SKIL
Plasmid#45289DepositorInsertSNON (SKIL Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-122a-5p
Plasmid#103183PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-122a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-122a-5p target (MIR122 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-126-5p
Plasmid#103193PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-126-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-126-5p target (MIR126 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85b(NC)-SH2
Plasmid#46468DepositorInsertp85b(NC) (PIK3R2 Bovine)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 311-717PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85b(C)-SH2
Plasmid#46466DepositorInsertp85b(C) (PIK3R2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 610-728PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K(S441A/S445A)
Plasmid#110161PurposeExpression of human eEF2K (S441A/S445A) in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationS441A/S445APromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-510-5p
Plasmid#103593PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-510-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-510-5p target (MIR510 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX tensin-SH2
Plasmid#46525DepositorInserttensin (TNS1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1451-1588PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Vrap-SH2
Plasmid#46531DepositorInsertVrap (SH2D2A Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 93-192PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(N)-SH2
Plasmid#46534DepositorInsertZap70(N) (ZAP70 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 1-116PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOD2049-csnControl
Plasmid#100007PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…TagsExpressionMutationPromoterAvailable sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tem6-SH2
Plasmid#46523DepositorInsertTem6 (TNS3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1171-1291PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant
Plasmid#72922PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationPromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S
Plasmid#34598DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationFragment 1781-2086 of KLF4PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only