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Plasmid#166703Purposeexpresses NEDD4L WW3 in bacterial cellsDepositorInsertNEDD4L WW3 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW4(548-581)
Plasmid#166704Purposeexpresses NEDD4L WW4 in bacterial cellsDepositorInsertNEDD4L WW4 domain
UseTagsGST, HRV 3C site, and Thrombin siteExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY153: Flag(SUZ12) WT HDR plasmid
Plasmid#170793PurposeWT homology directed repair plasmid for performing genome-editing on the SUZ12 LocusDepositorInsertSUZ12 (SUZ12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gLacZ.EFS-NS.H2B-RFP
Plasmid#170363PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb oligo-library based
Plasmid#158201PurposeCassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of QB based on oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH42
Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialMutationPromoternative promoter of sll0617Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd388-BPTF-sh28
Plasmid#83276PurposeshRNA targeting BPTFDepositorInsertshRNA to BPTF
UseAdenoviralTagsExpressionMutationPromoterpUC oriAvailable sinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-FLAG-SBP-STAG2 (Y1142fs)
Plasmid#73969PurposeSTAG2 cDNA (CCDS43990) Y1142fsDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationY1142fsPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (I885fs)
Plasmid#73966PurposeSTAG2 cDNA (CCDS43990) I885fsDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationI885fsPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (S202L)
Plasmid#73972PurposeSTAG2 cDNA (CCDS43990) S202LDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationS202LPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (V181M)
Plasmid#73971PurposeSTAG2 cDNA (CCDS43990) V181MDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationV181MPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1410
Plasmid#53011PurposepLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
ompC
rpoS
UseSynthetic BiologyTagsT7noRBS (900bp) and gfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1022
Plasmid#52996PurposepLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
OxyS sRNA
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1075
Plasmid#53004PurposepCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsfhlA
sodB
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1073
Plasmid#53003PurposepCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsompC (target mRNA) and sodB (target mRNA)ExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1394
Plasmid#53009PurposepLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
T7(st3) (133bps)
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only