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Plasmid#86843PurposeHR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.DepositorInsertFLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
UseTagsFLAG-HaloTagExpressionMammalianMutationPromoterEndogenous TERT promoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUDP004
Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRb delta CDK
Plasmid#58906Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removedDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationall 15 Ser/Thr Cdk acceptor sites changed to Ala,…PromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW-GFP-CtIP
Plasmid#71109Purposemammalian expession of GFP-CtlPDepositorInsertCtlP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKAR2-Br512
Plasmid#161875PurposeExpresses Br512 polymerase in E.coliDepositorArticleInsertModified DNA Polymerase I Large Fragment (Br512)
UseTags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialMutationPromoterT7, tetPAAvailable sinceNov. 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAM_Ec
Plasmid#102939PurposeTransposon mutagenesis and Tn-seqDepositorInsertmariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-KRAB
Plasmid#191314PurposepiggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFPDepositorInsertsKRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ADAM10-HA
Plasmid#65106PurposeExpression of HA tagged ADAM10 in mammalian cellsDepositorInsertADAM10 (ADAM10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSSRB069_pAC8-hsDDB1dB
Plasmid#218790PurposeInsect cell expression vector for 6xHis-TEV-hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTags6xHis-TEVExpressionInsectMutationDeleted amino acids 396-705 and replaced with GNG…PromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_MFE1
Plasmid#84612PurposeIntroduces DSB at MFE1 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCas9
Plasmid#41815PurposeExpresses human codon optimized Cas9 nuclease for genome engineeringDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOM20MTS-mCherry-EGFP-Tet-On
Plasmid#109016PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondriaDepositorInsertTOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DENV3_PrME
Plasmid#207265PurposeMammalian expression of Dengue virus 3 PrME proteinDepositorInsertDENV3_PrME (POLY Dengue virus 3)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DENV4_PrME
Plasmid#207266PurposeMammalian expression of Dengue virus 4 PrME proteinDepositorInsertDENV4_PrME (POLY Dengue virus 4)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRSin-IRES2-EmGFP_HPC-4 Ab light
Plasmid#187359PurposeLentiviral expression of HPC-4 antibody light chainDepositorInsertHPC-4 antibody light chain
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-WT-Highest-CAR_RHL004
Plasmid#164826PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CARDepositorInsertAntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-miRFP670nano-miRFP670
Plasmid#216131PurposeLentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.DepositorInsertEF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only