Showing: 7701 - 7720 of 20568 results
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Plasmid#167674PurposeIntermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSF280
Plasmid#167675PurposeIntermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRU325
Plasmid#167676PurposeIntermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJAT31
Plasmid#204291PurposeFor inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3AvailabilityAcademic Institutions and Nonprofits only -
pJAT58
Plasmid#204304PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1AvailabilityAcademic Institutions and Nonprofits only -
CMV-AsCas12f-WT
Plasmid#204635PurposeExpression of wild-type AsCas12f in mammalian cellsDepositorInsertAsCas12f
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CMV-enAsCas12f
Plasmid#204637PurposeExpression of enAsCas12f in mammalian cellsDepositorInsertenAsCas12f
UseTagsNLSExpressionMammalianMutationD196K, N199K, G276R, N328G, D364RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-AsCas12f-WT
Plasmid#204640PurposeExpression of wild-type AsCas12f in E. coliDepositorInsertAsCas12f
UseTags6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1
Plasmid#205152PurposeInducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRTDepositorInsertFRT-GFP-FRT-uORF-dCBEevoCDA1
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDGE1176
Plasmid#202774PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA
Plasmid#179211PurposeGolden Gate entry vector; 2nd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA
Plasmid#179212PurposeGolden Gate entry vector; 3rd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA
Plasmid#179213PurposeGolden Gate entry vector; 4th gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B
Plasmid#179216PurposeGolden Gate entry vector; 4th gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAtU3AvailabilityAcademic Institutions and Nonprofits only -
pORE303N
Plasmid#194438PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoter2x35SAvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-B
Plasmid#196987PurposeFor SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.DepositorInsertSpy gRNA cassette
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1AvailabilityAcademic Institutions and Nonprofits only -
pCDF_TypeIC_target_sfGFP_invivo
Plasmid#196399Purposeexpresses CRISPR Cascade Type IC target sequence for in vivo assayDepositorInserttype IC dsDNA target
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 7701 - 7720 of 20568 results