Addgene

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PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Use

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-mPar6a WT

Plasmid

#86148

Purpose

FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

Depositor

Senthil Muthuswamy

Article

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Par6 (Pard6a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 T179V

Plasmid

#27011

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST_EWSR1_C2G_R650G

Plasmid

#26382

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

R650G

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK8-His-VHL-Flag(117-213)

Plasmid

#29501

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Use

Tags

Flag and His

Expression

Insect

Mutation

VHL(117-213) only

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
Wnt8A STOP

Plasmid

#35879

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation A (600) --> G

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-myc-Rbx1

Plasmid

#29507

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Rbx1 (Rbx1 Mouse)

Use

Tags

myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
HOXB4-NA-MSCV

Plasmid

#20987

Depositor

Corey Largman

Article

Krosl et al Oncogene. 1998 Jul 2. 16(26):3403-12.

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N51A (makes DNA binding mutant protein)

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
#2.4 post-G-SynapShot-mScarlet

Plasmid

#214720

Purpose

Expresses green #2.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 post-G-SynapShot

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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mRFP-LAP2β

Plasmid

#228029

Purpose

lentiviral construct for mRFP-LAP2β expression

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

LAP2b (Tmpo Rat)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBsVchDonor

Plasmid

#203814

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

Mini-Tn, aprE cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TSen

Plasmid

#209916

Purpose

A FRET biosensor for ADAM17 activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TSen

Use

Tags

ECFP and YPet

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD_FLAG-GFP(Y39TAG)-6His

Plasmid

#223510

Purpose

Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Insert

GFP(Y39TAG)

Use

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

Y39TAG

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDLterm

Plasmid

#211903

Purpose

Base plasmid for dual-luciferase constructs to measure terminator strength

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

35S enhancer + 35S minimal promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Mutation

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Nm2Cas9-BlastR

Plasmid

#220980

Purpose

Expresses human codon-optimized Nm2Cas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

Nm2Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge