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Plasmid#157662PurposeExpresses API5 in mammalian cellDepositorInsertApoptosis inhibitor 5 (API5 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85a(C)-SH2
Plasmid#46463DepositorInsertp85a(C) (PIK3R1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 612-724PromoterTacAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p27 T187A Luc
Plasmid#23048DepositorInsertp27 (CDKN1B Human)
UseTagsLuciferaseExpressionMammalianMutationT187A: mutation at codon 187 changing from T (ACG…PromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179436PurposeDual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertHS4-TRE-cit-HS4-mch (DH)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Myc-tagged PSK
Plasmid#197149PurposeExpression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsMycExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn4a-NTD1-135-mNeon
Plasmid#186609PurposeEncodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreenDepositorInsertHuman Reticulon 4 isoform A amino acids 1 to 135 (RTN4 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOD2087-trnDEG
Plasmid#89369PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmec-18Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-2-3p
Plasmid#103144PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-2-3p target (MIRLET7A2 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-150-5p
Plasmid#103257PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-150-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-150-5p target (MIR150 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-CK2alpha’ V67A/I175A (pJD24)
Plasmid#37363DepositorInsertCK2α' V67A/I175A (CSNK2A2 Human)
UseTagsHAExpressionMammalianMutationV67A, I175APromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-BioID2-HA
Plasmid#130980PurposeExpression of HA-tagged KIF1C-BioID2DepositorInsertKIF1C-BioID2-HA (KIF1C Human)
UseTagsBioID2-HAExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-223-3p
Plasmid#103369PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-223-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-223-3p target (MIR223 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-3p
Plasmid#103365PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-3p target (MIR221 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Cis1-SH2
Plasmid#46417DepositorInsertCis1 (SOCS1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 90-214PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187950PurposeecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
J23117-mRFP1-331Bb
Plasmid#78280PurposemRFP1 behind constitutive promoter J23117 in pSEVA331Bb backboneDepositorInsertJ23117-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG II-BTLA-mGFP
Plasmid#180798PurposeExpression of mouse BTLA fused to mGFPDepositorInsertBTLA (Btla Mouse)
UseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG II SIN-SV40-mCherry-SHP1
Plasmid#180799PurposeExpression of mouse SHP1 fused to N-terminal mCherryDepositorInsertSHP1 (Nr0b2 Mouse)
UseRetroviralTagsExpressionMutationPromoterSV40Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only