Addgene

We narrowed to 18,521 results for: omp

Showing: 7641 - 7660 of 18521 results

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCBa

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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SaABE8e-dimer

Plasmid

#138501

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSaCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-AdenoX

Plasmid

#122123

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Use

Adenoviral

Tags

Omni

Expression

Mutation

Promoter

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-F63Q

Plasmid

#104363

Purpose

Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

mutated the following amino acid: F63Q

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Vav1-SH2

Plasmid

#46528

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vav1 (VAV1 Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 661-777

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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enAsABE8e-dimer

Plasmid

#138507

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SLA2-1/1

Plasmid

#91450

Purpose

Protein expression and purification of human SH3 domain construct SLA2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SLA2-1/1 (SLA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-2/3

Plasmid

#91362

Purpose

Protein expression and purification of human SH3 domain construct NCK2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-2/3 (NCK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSR353

Plasmid

#125078

Purpose

Expression of narL under PLtetO-1, output promoter PdcuS77

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL (narL )
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTre2tet)::Fbxl21

Plasmid

#51890

Purpose

expression of Fbxl21

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV tet

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b

Plasmid

#52122

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
myc-hIFITM3-delta59-68

Plasmid

#104361

Purpose

Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

deleted amino acids 59 through 68

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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RAMA-bio-His

Plasmid

#50737

Purpose

Expresses enzymatically monobiotinylated full length RAMA ectodomain

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

RAMA

Use

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin3

Plasmid

#136807

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB5

Plasmid

#19527

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Use

Tags

Expression

Insect

Mutation

P418S

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOpLacI:mini35ss (GB0534)

Plasmid

#68179

Purpose

Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xOpLacI:mini35S

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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