Addgene

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Use

Tags

Expression

Insect

Mutation

P418S

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Use

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1160

Plasmid

#179298

Purpose

Expresses dCas9 fused to P65

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-p65

Use

Tags

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-Venus

Plasmid

#174472

Purpose

Express Venus with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with golgi apparatus-targeting sequence

Use

Tags

β1,4-galactose transferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-Venus

Plasmid

#174187

Purpose

Express Venus with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with mitchondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt8A-V5

Plasmid

#35935

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (600) --> G

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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dmBACCS2NS-IRES-dOrai

Plasmid

#67628

Purpose

Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2NS-IRES-dOrai

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pTYF-1xGfaABC1D-tTA

Plasmid

#19977

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

GfaABC1D-tTA (GFAP Human, E.coli)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52537

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

Plasmid

#52538

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSW179 - pML1

Plasmid

#115977

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Use

Tags

His and myc

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-ABE

Plasmid

#107723

Purpose

For expression of ABE7.10 in mammalian cells

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Insert

ABE7.10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Ares-Aphrodite

Plasmid

#13571

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Use

Tags

Aphrodite and Ares

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk2 DN HA

Plasmid

#27651

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

D146N (Dominant Negative)

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pmCellFree _KA2_ZIKV NS5

Plasmid

#145368

Purpose

Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

Plasmid

#69224

Purpose

Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS and Zif268

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-Y100F

Plasmid

#110826

Purpose

Bacterial expression of E.coli DHFR Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

Y100 mutated to F100

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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