Addgene

We narrowed to 13,597 results for: pET

Showing: 7621 - 7640 of 13597 results

Results per page:

Sort results by:

CFAST-Halotag-ER

Plasmid

#214413

Purpose

ER localization of CFAST fused to Halotag-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

rat cb5 ERM

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NFAST(low)-Halo-ER

Plasmid

#214419

Purpose

ER localization of low-affinity NFAST fused to Halo-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NFAST(low)-mApple-mito

Plasmid

#214415

Purpose

Mitochondria localization of low-affinity NFAST fused to mApple-mito

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

mApple-mito

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NFAST(low)-mApple-6xHp

Plasmid

#214412

Purpose

Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Use

Tags

mApple-6xHp

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GL4.10 Prg4 promoter-luciferase

Plasmid

#195034

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter (PRG4 Bovine)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-His6

Plasmid

#212780

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-yeGFP

Plasmid

#212769

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(63)-VSV

Plasmid

#212753

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(48)-VSV

Plasmid

#212744

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(48)-VSV

Plasmid

#212743

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NUbo-E3(63)-FLAG

Plasmid

#212808

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Tags

FLAG and NUbo

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NUbo-FLAG

Plasmid

#212797

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K48R/K63R)-yeGFP

Plasmid

#212774

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-yeGFP-His6

Plasmid

#212785

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-9myc

Plasmid

#212789

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K48R/K63R)

Plasmid

#212765

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge