Addgene

We narrowed to 18,521 results for: omp

Showing: 7621 - 7640 of 18521 results

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pAPM-D4 miR30-SETDB1 ts2

Plasmid

#115860

Purpose

SETDB1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SETDB1 shRNA (SETDB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xFKBP

Plasmid

#20221

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFKBP (FKBP1A Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmAGO1

Plasmid

#22420

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pIBA2-SpyTag-MBP

Plasmid

#107422

Purpose

Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

Maltose-binding protein (MalE)

Use

Tags

OmpA signal peptide, SpyTag, and StrepII

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

Plasmid

#99679

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64-p65(101-261)-RTA(125-190)

Expression

Mutation

dead Cas9

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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DYNLT1

Plasmid

#132528

Purpose

Human TCTEX-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT1 (DYNLT1 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1-ATG17-3'UTR

Plasmid

#166848

Purpose

Expresses Atg1 with ATG17 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

ATG1 Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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Vh-HA-3xFKBP

Plasmid

#20222

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
mr in pUASP

Plasmid

#112981

Purpose

P element vector for transposon insertion of mr (APC2) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kashevsky et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

mr (APC2) (mr Fly)

Use

P insertion vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTHsp18.2 (GB0035)

Plasmid

#68186

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_508-SV40-GFP

Plasmid

#75138

Purpose

Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_508

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pBS U6 WIP RNAi

Plasmid

#11774

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP RNAi (WIPF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pICSL11023

Plasmid

#117542

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S_OMEGA(pICH51288)

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-WRC-vpr

Plasmid

#115839

Purpose

Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVWRC Vpr (AM713177)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GOR -vpr

Plasmid

#115835

Purpose

Encodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGOR Vpr (FJ424871)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VERAGM3 vpr

Plasmid

#115826

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver AGM3) (M30931)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SAB-92018 vpr

Plasmid

#115822

Purpose

Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSAB Vpr (HQ378594)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Syk(C)-SH2

Plasmid

#46519

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(C) (SYK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 149-263

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

Plasmid

#81206

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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