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Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2ExpressionMutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Red cGull
Plasmid#191853PurposeExpresses biosensor for cGMP in mammalian cellsDepositorInsertRed cGull
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 BLAST
Plasmid#164228Purposelentiviral plasmid for GFP-hTREX1DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
UseTagsHis-LipoylExpressionBacterialMutationPromoterT7Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#63154PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLSDepositorInsertCas9
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA
Plasmid#140221PurposeConstitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipperDepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-H2B-mEGFP
Plasmid#105528PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNADepositorInsertH2B (H2BC11 Human)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔPRYSPRY
Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔRING-Box
Plasmid#105520PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationdeletion of RING domain (aa124-462)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔRING-Box
Plasmid#105523PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryExpressionMutationdeletion of RING domain (aa124-462)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLBD2-6xHis S392A
Plasmid#213607PurposeExpresses PLBD2-6xHis S392A in mammalian cellsDepositorInsertphospholipase B domain containing 2 (PLBD2 Human)
UseTags6xHisExpressionMammalianMutationChanged serine 392 to alaninePromoterCMVAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Puro-TCPTP
Plasmid#33366DepositorInsertTCPTP-45 (PTPN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3AmaxNG-BlastR
Plasmid#152999PurposeC-to-T base editor, NG PAMDepositorInserteA3A-nSpCas9 NG-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3Amax-BlastR
Plasmid#152997PurposeC-to-T base editorDepositorInserteA3A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-BE4max-BlastR
Plasmid#152991PurposeC-to-T base editorDepositorInsertBE4-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Muc2D1D2D3
Plasmid#155215PurposeExpresses human MUC2 residues 21-1259DepositorInsertMuc2 D1D2D3 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-ABEmaxCP1041-BlastR
Plasmid#152990PurposeA-to-G base editorDepositorInsertABE7.10-nSpCas9 CP1041
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)
Plasmid#191139PurposeExpression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gatesDepositorInsertt17d9b
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140229PurposeDox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFPDepositorInsertAntiCRISPR protein AcrVA1
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only