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Plasmid#46778Purposeexpression of N124I Rab1a in mammalian cellsDepositorInsertRab1a (Rab1a Mouse)
UseTagsFLAGExpressionMammalianMutationN124I cannot stably bind guanine nucleotidePromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
UseTags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e-dimer
Plasmid#138503PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588D
Plasmid#48194PurposeExpresses L588D mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588A
Plasmid#48193PurposeExpresses L588A mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_R665A
Plasmid#48195PurposeExpresses R665A mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slap-SH2
Plasmid#46500DepositorInsertSlap (SLA Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 61-221PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-2/3
Plasmid#91420PurposeProtein expression and purification of human SH3 domain construct NCK1-2/3DepositorInsertNCK1-2/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginC
Plasmid#29505DepositorInsertElongin C (ELOC Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKT3Ts-goldyTALEN
Plasmid#80330PurposeUpdated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequenceDepositorTypeEmpty backboneUseTALENTagsExpressionBacterialMutationPromoterT3Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SUV39h1 ts1
Plasmid#115880PurposeSUV39h1 knockdownDepositorInsertSUV39h1 shRNA (SUV39H1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-myc
Plasmid#20212DepositorInsert2xFKBP (FKBP1A Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLuciferase (GB0096)
Plasmid#68201PurposeProvides the CDS of the firefly luciferase protein as a level 0 GoldenBraid partDepositorInsertLuciferase coding region
UseLuciferase and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat4-SH2
Plasmid#46514DepositorInsertStat4 (STAT4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 133-678PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-PPHLN1 ts3
Plasmid#115879PurposePPHLN1 knockdownDepositorInsertPPHLN1 shRNA (PPHLN1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MPHOSPH8 ts1
Plasmid#115874PurposeMPHOSPH8 knockdownDepositorInsertMPHOSPH8 shRNA (MPHOSPH8 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SETDB1 ts3
Plasmid#115861PurposeSETDB1 knockdownDepositorInsertSETDB1 shRNA (SETDB1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only