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Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (F317D)
Plasmid#121406PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (I325D)
Plasmid#121407PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC176
Plasmid#118891Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; SNAP, cleav…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS2
Plasmid#113091PurposeLuciferase reporter containing PAK4 3' UTR with the second predicted MiR-199a-3p bindingDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationnonePromoterPGKAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.a-AP-His
Plasmid#72054PurposeExpresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5c.a (Unc5c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Neo1.e-Fc-His
Plasmid#72093PurposeExpresses the extracellular region of the Neogenin 1, isoform e protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNeo1.e (Neo1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _1336-1496
Plasmid#86823Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _1521-2092
Plasmid#86824Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-3'UTR-BS1
Plasmid#113090PurposeLuciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding siteDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationnonePromoterPGKAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302 PCSK5 (T288P)-V5 puro
Plasmid#98709PurposeLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Threonine 288 to ProlinePromoterAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_M337P
Plasmid#98675PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with M337PDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationM337PPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A326P
Plasmid#98672PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A326PDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationA326PPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _2095-2257
Plasmid#86825Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only