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Showing: 741 - 760 of 13195 results

  1. RFN_EGFP_82

    Plasmid
    #60074
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. RFN_EGFP_80

    Plasmid
    #60072
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  6. pPEE47

    Plasmid
    #139426
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    139426-plasmid-map-sequence-id-297721

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  7. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

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  9. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  17. Cre-responsive Cas9 expressing transposon library vector

    Plasmid
    #164138
    Purpose
    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, EF-1a
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    164138-plasmid-map-sequence-id-333975

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Showing: 741 - 760 of 13195 results