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Plasmid#217701PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG3 (ADGRG3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-249PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG1 CTF-FLAG
Plasmid#217699PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG1 (ADGRG1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-382PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG2 CTF-FLAG
Plasmid#217700PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG2 (ADGRG2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-606PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF1 CTFdeltaTA-FLAG
Plasmid#217727PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-572PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG
Plasmid#217735PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG4 (ADGRG4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2727PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG
Plasmid#217734PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG3 (ADGRG3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-255PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG
Plasmid#217738PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG7 (ADGRG7 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-421PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG
Plasmid#217723PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-523PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG
Plasmid#217733PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG2 (ADGRG2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-612PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
UseTagsExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-GST-TEV-mCherry-NAP1 (WT)
Plasmid#198036PurposeUsed for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)DepositorInsertNAP1 (AZI2 Human)
UseTagsGST-TEV and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMSr-TEV-Twin-Strep
Plasmid#202530PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cellDepositorInsertSAMD8 (SAMD8 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tev-IntC-P53(304-393)
Plasmid#200315Purposebacterial expression of P53 TETCTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6GB1tev and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
Plasmid#198037PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TEV-mCherry-TBK1
Plasmid#198033PurposeUsed for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV and mCherryExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA
Plasmid#194367PurposeSplit TEV assaysDepositorInsertSP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA
Plasmid#194368PurposeSplit TEV assaysDepositorInsertHTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only