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Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-X(4130)B1
Plasmid#168018PurposeExpresses X(4130)B1-6xHis in bacterial cellsDepositorInsertX(4130)B1-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Yuh1
Plasmid#18895DepositorInsertUbiquitin C-terminal hydrolase I (YUH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PSAT
Plasmid#74115PurposeExpression of PSATDepositorInsertpET28a-PSAT (PSAT1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIRT5-pET15b
Plasmid#81030PurposeBacterial expression of Human WT SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-hlPYK WT
Plasmid#187975PurposeExpresses the human liver pyruvate kinaseDepositorInserthlPYK WT
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN-01*
Plasmid#80423PurposeFor expression of EPN-01* in E. coliDepositorInsertEPN-01*
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIRT5H158Y-pET15b
Plasmid#81032PurposeBacterial expression of Human catalytic mutant SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
UseTagsHisExpressionBacterialMutationH158Y catalytic mutantPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-LahT150
Plasmid#166785PurposeCleavage of double glycine motif in peptides to remove the leader sequenceDepositorInsertLahT150
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-K48C
Plasmid#18897DepositorInsertUbiquitin-K48C (UBC Human)
UseTagsExpressionBacterialMutationLysine 48 is replaced with a Cysteine residuePromoterAvailable sinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET11a+CYP119
Plasmid#66131PurposeExpresses the wild type of CYP119DepositorInsertCYP119 (cyp119 )
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-AldA WT
Plasmid#187976PurposeExpresses human aldolase ADepositorInsertAldA WT (ALDOA Human)
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
YPet-H2B-6
Plasmid#56632PurposeLocalization: Histone H2B, Excitation: 513, Emission: 527DepositorInsertH2B (H2BC11 Human)
UseTagsYPetExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SznE
Plasmid#139282Purposeexpresses SznE in E. coliDepositorInsertsznE
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478, 20-62PromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28b::mAID
Plasmid#15923DepositorInsertAID (Aicda Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH45
Plasmid#199767PurposeExpresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH45
UseTagsHis6-TagExpressionBacterialMutationinternal His-TagPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-pA-Mnase
Plasmid#124883PurposeTo transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.DepositorInsertprotein A-MNase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ec_bPAC
Plasmid#28135PurposeExpression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coliDepositorInsertE.coli adapted photoactivated adenylyl cyclase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only