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Plasmid#115918Purposedonor to insert IRES-EGFP at the GAPDH locus in human cellsDepositorInsertIRES-EGFP
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe3-WT
Plasmid#85847PurposeDonor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomatoDepositorInsertSNCA exon 3 homology arms (SNCA Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
UseTagstdTomato-v5-GCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-ChrimsonR-tdT
Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorInsertChrimsonR-tdTomato
UseTagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT
Plasmid#59171PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-fwd[Chrimson-tdT]
Plasmid#59137PurposeExpression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)DepositorInsertChrimson-tdTomato
UseCre/LoxTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptdTHC_PGKneoLox2DTA.2
Plasmid#112625PurposeH2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagstdTomatoExpressionMutationPromoterNo PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tdTHC
Plasmid#112621Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2DepositorInsertsUseCre/LoxTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptdTHC
Plasmid#112617Purposepromoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2DepositorInsertsUsePromoterless vector, ready for promoter for mamma…TagstdTomatoExpressionMammalianMutationPromoterNo PromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2K7-RIP-Tomato-EFs-Tk::Sh
Plasmid#114311PurposeTissue-specific expression of the tdTomato living color under the control of the Rat Insulin 2 promoter (RIP)DepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-BnTW
Plasmid#133365PurposePiggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLSDepositorInserttdTomato-NLS
UseTagsFlag-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PnTW
Plasmid#133356PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLSDepositorInserttdTomato-NLS
UseTagsHA-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili-Luc
Plasmid#48688Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP-CoV2/stSpike-puro
Plasmid#161794PurposeTransposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein productionDepositorInsertSARS-CoV-2 secretable trimeric Spike
UseTransposonTags6xHis and dTomatoExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP-CoV2/RBD-puro
Plasmid#161793PurposeTransposon-based SARS-CoV-2 RBD stable expression vector for protein productionDepositorInsertSARS-CoV-2 RBD
UseTransposonTags6xHis and dTomatoExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato
Plasmid#138610PurposeExpress TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backboneDepositorInsertsHyperactive AID
T7 RNA Polymerase
tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only