Skip to main content
Addgene
Showing: 741 - 760 of 3847 results

  3. pFP.R400

    Plasmid
    #138175
    Purpose
    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138175-plasmid-map-sequence-id-296599

    Enlarge

  4. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Use
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pUM31dao

    Plasmid
    #87242
    Purpose
    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants
    Depositor
    Anil Day
    Article
    Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.
    Insert
    D-amino oxidase
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    plastid rrn
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    87242-plasmid-map-sequence-id-211979

    Enlarge

  9. NODALvar-CAG-AAVS1

    Plasmid
    #115262
    Purpose
    For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115262-plasmid-map-sequence-id-222505

    Enlarge

  11. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138245-plasmid-map-sequence-id-273498

    Enlarge

  14. pFP.R271

    Plasmid
    #138220
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138220-plasmid-map-sequence-id-296583

    Enlarge

  17. pFP.R365

    Plasmid
    #138235
    Purpose
    Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138235-plasmid-map-sequence-id-273528

    Enlarge

  18. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid
    #70229
    Purpose
    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Use
    Tags
    3XFLAG, FK506- and rapamycin-binding protein (FKB…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. psiT-GEM(0)

    Plasmid
    #220446
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220446-plasmid-map-sequence-id-443761

    Enlarge

Showing: 741 - 760 of 3847 results