-
Plasmid#123221PurposeN-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
1455_pDEST_miniTol2_R4-R2_MCS
Plasmid#171794PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpic
Plasmid#84372Purpose3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARS-CoV-2 S1 RBD
Plasmid#167013PurposeGateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseTagsExpressionMammalianMutationNo stop codonPromoterAvailable sinceApril 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR P2R-P3-EGFP
Plasmid#48349PurposeContributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationAminoacid 40 is valine in our sequence, while it …PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-V5-6xHis
Plasmid#48347PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a stop codon at the end of the epitope t…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EGFP
Plasmid#48348PurposeContributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-V5-6xHis
Plasmid#48346PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a Kozak sequence and initiation codon up…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-dr_Unc (JDW 1365)
Plasmid#224482PurposeGateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intronDepositorInsertUnc-503 promoter
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME V5 mScarlet-I (JDW 1323)
Plasmid#224487PurposeGateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)DepositorInsertV5-mScarlet-I
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-myr-mTagBFP2-Flag (JDW 1183)
Plasmid#224492PurposeGateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)DepositorInsertmyr-mTagBFP2-Flag
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-mCherry-3xHA (JDW 1321)
Plasmid#224493PurposeGateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)DepositorInsertLifeact-mCherry-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-EGFP-3xHA (JDW 1322)
Plasmid#224494PurposeGateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)DepositorInsertLiefact-EGFP-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Actin-Vhh sfGFP (JDW 1215)
Plasmid#224495PurposeGateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)DepositorInsertActin-Vhh-sfGFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mCherry (JDW 1240)
Plasmid#224499PurposeGateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mCherry
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet3-Giantin (JDW 1356)
Plasmid#224502PurposeGateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)DepositorInsertmScarlet3-Giantin
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mCerulean3-TUBB1A (JDW 1367)
Plasmid#224503PurposeGateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cellsDepositorInsertmCerulean3-TUBB1A
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-TAEL (JDW 1324)
Plasmid#224508PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)DepositorInsertTAEL
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS1 (JDW 1095)
Plasmid#224510PurposeGateway compatible middle entry clone containing a Myc-tagged BIOID2 constructDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only