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Plasmid#182887PurposeTransfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsEGFPExpressionBacterial and MammalianMutationsilet mutations in aa 197 - 203 to confer resista…PromoterEF1alpha coreAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D665A pcDNA3
Plasmid#206113Purposeexpression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functionalDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationD665APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1A KI
Plasmid#131480PurposeEndogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
UseTagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1E KI
Plasmid#131481PurposeEndogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA L702P pcDNA3
Plasmid#206056Purposeexpression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functionalDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationL702PPromoterCMVAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA L702 P pCAGGS
Plasmid#206055Purposeexpression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functionalDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationL702PPromoterCMV/B-actinAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Nck1 (SH2 mut)
Plasmid#45893DepositorInsertNck-1 SH2 mutant (NCK1 Human)
UseTagsEGFPExpressionMammalianMutationin SH2 domain (R308K) and E302K and Q333HPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTBL3328 PB-sfGFP-WPRE-target
Plasmid#226676PurposePiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.DepositorInsertsfGFP-WPRE-site3target
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP)
Plasmid#172339PurposemnucTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pegfp-N1-TFEB-S3A,R4A
Plasmid#44446DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationChanged both Serine 3 and Arginine 4 to AlaninePromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Gria3-GFP KI
Plasmid#131491PurposeEndogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-RPA194
Plasmid#179237Purposeoverexpression in human cellsDepositorInsertRPA194 (POLR1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN-deltaNLS3
Plasmid#22158DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationDeletion of amino acids 883-889PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN-deltaSS
Plasmid#22159DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationDeletion of amino acids 897-908PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-GFPi
Plasmid#31849DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherryAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 cyto Grx1-roGFP2
Plasmid#64999PurposeExpression of cytosolic Grx1-roGFP2 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-FBL
Plasmid#179235Purposeoverexpression in human cellsDepositorInsertFBL (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only