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Plasmid#35136DepositorInsertHPV57 E7
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pX330_human CDK1
Plasmid#118597PurposeOne shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.DepositorInsertguide RNA against human CDK1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMZ-178
Plasmid#195426PurposeTransient, constitutive expression of synZiFTR: ZF3-p65DepositorInsertpUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-185
Plasmid#195433PurposeTransient, constitutive expression of synZiFTR: ZF10-p65DepositorInsertpUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate-VP64
Plasmid#47389PurposeExpression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFLAG, HA, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-UGI-nCas9(H840A)-GSVG
Plasmid#187411PurposeDddA GSVG variantDepositorInsertDddA GSVG variant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-Myc
Plasmid#214722PurposeExpresses green #2.4 pre-SynapShot and Myc tagDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad-mCherry-XL (R125K)
Plasmid#188054PurposeExpresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteriaDepositorInsertmCherry-XL (R125K)
UseTags6xHisExpressionBacterialMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoteraraBADAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
#2.4 pre-G-SynapShot-TagBFP
Plasmid#214721PurposeExpresses green #2.4 pre-SynapShot and TagBFPDepositorInsert#2.4 pre-G-SynapShot
UseAAVTagsTagBFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0045-EF1a-PguCas13b-NES-HIV
Plasmid#103861PurposeExpresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPguCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-NRF1-HA
Plasmid#34708DepositorInsertNRF1 (NFE2L1 Human)
UseRetroviralTags3XFlag and HAExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cytb5
Plasmid#182579PurposeExpresses mCherry targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertmCherry-Cytb5
UseTagsCytb5ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mADAM17 in pcDNA3.1
Plasmid#19141DepositorInsertADAM17 (Adam17 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro NFS1
Plasmid#102977PurposeExpression of NFS1DepositorInsertNFS1 (NFS1 Human)
UseRetroviralTagsExpressionMammalianMutationSilent mutations to eliminate shNFS1_1 binding si…PromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-4X(MS2-PP7)
Plasmid#121941PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-4X(MS2-PP7)
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
HBT-GCaMP6-HA
Plasmid#107433PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertGCaMP6S
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only