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Plasmid#72182PurposeExpresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5c.z (Unc5c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sdk2-AP-His
Plasmid#72009PurposeExpresses the extracellular region of the Sdk2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSdk2 (Sdk2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb3-AP-His
Plasmid#72004PurposeExpresses the extracellular region of the PlexinB3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb3 (Plxnb3 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.z-Fc-His
Plasmid#72185PurposeExpresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5d.z (Unc5d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL417
Plasmid#62822PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1DepositorInsertsgfp
micC leader
hfq leader
UseSynthetic BiologyTagsompC leader and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf111
Plasmid#12799PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf111
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4CTF-PY1 mutant
Plasmid#17798DepositorInsertErbB4 (ERBB4 Human)
UseTagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment, amino acids 676-1292, …PromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf270
Plasmid#12958PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf270
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pET21a-TEX264_wt-His6
Plasmid#220214PurposeBacterial expression of wild type TEX264, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB143
Plasmid#222214PurposeFor recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tagDepositorInsertvanB(A24P)
UseTagsN-terminal, thrombin-cleavable 6x His tagExpressionBacterialMutationChanged Alanine 24 to ProlinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-haA3A-CBE-G-C
Plasmid#220899PurposeAAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNADepositorInserthaA3A-CBE-G-C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s pc3901
Plasmid#201700PurposeExpresses of GFP-tagged mTet1s in mammalian cellsDepositorInsertTet1s (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-mTet1s pc3905
Plasmid#201703PurposeExpresses of mcherry-tagged mTet1s in mammalian cellsDepositorInsertTet1s (Tet1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn PGK1-HALO
Plasmid#220910PurposeNeuronal specific expression of the fusion construct PGK1-HALODepositorInsertPGK1-HALO (PGK1 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 myc-Rab10-mito
Plasmid#208367PurposeMammalian expression of myc-tagged human Rab10 with C-terminal mito signalDepositorInsertmyc-Rab10-mito (RAB10 Human)
UseTagsExpressionMammalianMutationwildtypePromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro
Plasmid#189634PurposeLentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs librariesDepositorInserthU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 360-684
Plasmid#205055PurposeTWNK protein expressionDepositorInsertTWNK (TWNK Human)
UseTagsExpressionBacterialMutationLacks N terminal amino acid from 1-359PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EYFP Cav2.2 pRK5
Plasmid#206099Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASPIre4
Plasmid#196656PurposeDerivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.DepositorInsertBxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
UseTagsExpressionBacterialMutationSpeI site in Bxb1 CDSPromoterrhamnose-inducible promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only