-
Plasmid#192132PurposeExpresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance geneDepositorInsertHsp1-Hsp2Cas9-Y
UseAAVTagsExpressionMammalianMutationHsp1-Hsp2Cas9 (Y446A)PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ E. coli, Synthetic)
UseExpression of nuclear lacz reporterTagsNuclear localization signalExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-HistoneH2B-L2
Plasmid#186357PurposeEntry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertHistone H2B (H2BC11 Human)
UseExpression of a tagged nuclear markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_047
Plasmid#180551PurposeEntry vector containing sfGFP in E1.3a formatDepositorInsertsfGFP in E1.3a format
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_110
Plasmid#180554PurposeEntry vector containing 7xhis tag in E1.3b formatDepositorInsert7xhis tag in E1.3b format
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_007
Plasmid#180563PurposeEntry vector containing Terminator sequence (Bba_B0015)DepositorInsertTerminator sequence (Bba_B0015)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_044
Plasmid#180532PurposeEntry vector containing J23117 anderson promoterDepositorInsertJ23117 anderson promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_043
Plasmid#180531PurposeEntry vector containing J23101 anderson promoterDepositorInsertJ23101 anderson promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_031
Plasmid#180556PurposeEntry vector containing Terminator - BBa_B0010 terminatorDepositorInsertTerminator - BBa_B0010 terminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_032
Plasmid#180528PurposeEntry vector containing pLac promoterDepositorInsertpLac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_045
Plasmid#180543PurposeEntry vector containing RBS 30,000 a.u. designed strengthDepositorInsertsRBS 30,000 a.u. designed strength
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_034
Plasmid#180526PurposeEntry vector containing pLux promoterDepositorInsertpLux promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_252
Plasmid#180553PurposeEntry vector containing mScarlet in E1.3b formatDepositorInsertmScarlet in E1.3b format
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_046
Plasmid#180544PurposeEntry vector containing RBS 250 a.u. designed strengthDepositorInsertsRBS 250 a.u. designed strength
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_042
Plasmid#180530PurposeEntry vector containing J23100 anderson promoterDepositorInsertJ23100 anderson promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 ZUC
Plasmid#169964PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-MCS-LexA-VP16-MiniWhite
Plasmid#165900PurposeBlasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-P2A-APEX2-VHH[GFP]
Plasmid#126749PurposeExpression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJDepositorInsertVHH[EGFP]
UseZebrafish muscle expression cloneTagsmKate2-P2A-APEX2ExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND L370G
Plasmid#169970PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationL370GPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddtoxA-C
Plasmid#171724PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only