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Plasmid#115881PurposeSUV39h1 knockdownDepositorInsertSUV39h1 shRNA (SUV39H1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBM0090
Plasmid#172824PurposeLevel 2 module containing mCHERRY-nVENUS HSP21-cVENUSDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1
Plasmid#166711Purposeexpresses AMOT with inactivating mutation in first PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 L129A P130A
UseTags2xHAExpressionMammalianMutationchanged leucine 129 to alanine, proline 130 to al…PromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
YEpURA-CUP1-HBTubiquitin
Plasmid#26900DepositorInsertHBT-tagged ubiquitin
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre_59.F2
Plasmid#60207PurposeExpresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
UseTagsFKBP12 and Nuclear localisation signalExpressionMammalianMutationamino acids 19-59 of the humanized version of Cre…PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
CP1041-ABE8e-dimer
Plasmid#138499PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 HA-PNRC1
Plasmid#123294PurposeMammalian expression of PNRC1 N-HADepositorInsertPNRC1 (PNRC1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS U6 WASp RNAi
Plasmid#11775DepositorInsertWASp RNAi (WAS Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-Flag-ZO-2
Plasmid#27420DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2
Plasmid#127360Purposegateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-851 A50I mutant
Plasmid#46269DepositorInsertVcl 1-851 A50I mutation (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-851 (Vh) head domain, A50I mutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-FOP
Plasmid#136796PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tagDepositorInsertSNAP-FGFR
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD280
Plasmid#125071PurposeExpression of SO_4387 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-Meis1a
Plasmid#78144Purposebacterial expression of GST-Meis1aDepositorInsertMeis1a (Meis1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2-TEV-YFP-Flag
Plasmid#41004DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsTEV-YFP-FlagExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean-WW-NES
Plasmid#84629PurposeEncodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertCerulean-WW-NES
UseTags6xHis, Cerulean, Nuclear export signal (NES), T7 …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHis-hPim1
Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
UseTagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-dNme2Cas9
Plasmid#193190PurposeLentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)DepositorInsertdNme2Cas9
UseTagsNLS and NLS-3xHA-NLSExpressionMammalianMutationH588A, D16APromoterSFFVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only