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Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV HA TrkB
Plasmid#39978DepositorInsertTrkB (Ntrk2 Rat)
UseTagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-phiC31-LP-TC
Plasmid#183755PurposeAAVS1 targeting construct with the phiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
UseTags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Membrane protein gRNA library
Pooled Library#113345PurposeContains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAsDepositorSpeciesHomo sapiensUseLentiviralAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChrimsonR-tdTomato
Plasmid#124231PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomatoDepositorInsertChrimsonR-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE-FLP
Plasmid#45978DepositorInsertflp
UseSynthetic BiologyTagsExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)
Viral Prep#100849-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (#100849). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs
Plasmid#106908Purposefull-length murine SSeCKs/AKAP12/Gravin in retroviral vectorDepositorInsertSSeCKs (Akap12 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromotergagAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8s
Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1
Plasmid#72266PurposeExpress gene of interest under EF-1 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK-FLAG-UbxD8
Plasmid#53777Purposeexpress FLAG-tagged human UbxD8 geneDepositorInsertUbxD8 (FAF2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Leo1
Plasmid#11061DepositorInsertLeo1 (LEO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Leo1
Plasmid#11061DepositorInsertLeo1 (LEO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TfR-OVA
Plasmid#64600PurposeExpression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentationDepositorInsertOvalbumin
UseTagsTransmembrane domain of Transferrin Receptor (TfR…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR8-YFP
Plasmid#13024DepositorInsertToll-Like Receptor 8 (TLR8 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only