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Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPT107
Plasmid#85525PurposeRepressilator with triple reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
mKate2
UseSynthetic BiologyTagsssrAExpressionBacterialMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVDM1001
Plasmid#134660PurposeCRISPR delivery and repair template delivery vectorDepositorInsertCRISPR guide RNA array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin
Plasmid#134863PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Mito-Car-GECO
Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-L
Plasmid#120161PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 Lnp-mCherry
Plasmid#86687PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertLNPK (LNPK Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-EGFP
Plasmid#51093PurposeForebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled EGFP fluorophoreDepositorInsertoChIEF(E163A/T199C)
UseAAVTagsP2A-EGFPExpressionMammalianMutationE163A/T199CPromoterCaMKIIaAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
ADSL_pLX307
Plasmid#98311PurposeLentiviral expression of ADSLDepositorInsertADSL (ADSL Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
Plasmid#71811PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
XOR gate-V2.0
Plasmid#44453DepositorInsertXOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2890
Plasmid#142168PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-AMP-Bv16SrRNA
Plasmid#218518PurposepUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantificationDepositorInsert16S rRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIP-Flag-TfebAA
Plasmid#79014PurposeFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.DepositorInsertTfeb (Tfeb Mouse)
UseTagsFlagExpressionMammalianMutationS142A, S211APromoterpCAGGsAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only