-
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
HL 3325 (=HL 770 + rhyB knockout_KanR)
Bacterial Strain#30065DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
UseTagsExpressionBacterialMutationPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP WT
Plasmid#197567PurposeExpression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP 150TAG
Plasmid#197568PurposeExpression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS GFP (CT#22)
Plasmid#13878DepositorInsertGFP
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
RISeGFP (CC#353)
Plasmid#16131DepositorInserteGFP
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRIAS (CC#15)
Plasmid#15147DepositorTypeEmpty backboneUseRetroviral; Avian expressionTagsgag and polExpressionMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSLIRES-11 (CC#13)
Plasmid#15145DepositorTypeEmpty backboneUseAdapter vector for cloning into prisapTagsIRESExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
DH5alpha::DddI
Bacterial Strain#179631PurposeFor expression of toxic DddADepositorBacterial ResistanceGentamicinAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Axin/pCS2HA
Plasmid#16305DepositorInsertAxin (axin1.L Frog)
UseVertebrate expressionTagsHAExpressionMutationPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRISAP (CC#8)
Plasmid#15142DepositorTypeEmpty backboneUseRetroviral; Avian expressionTagsPLAP, gag, and polExpressionMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ma-Barnase
Plasmid#197572PurposeNegative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertsBarnase - 2xTAG
M. alvus Pyl-tRNA (6)
UseTagsnoneExpressionBacterialMutation3TAG and 45TAGPromoteraraC and lppAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ma-pREP
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
UseTagsExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SBECN1W
Plasmid#107937PurposeAdeno-Associated viral (AAV) vector plasmid expressing mouse Beclin-1 (BECN1) in neuronsDepositorInsertmouse Beclin-1 (BECN1) (Becn1 Mouse)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP 150TAG
Plasmid#197570PurposeExpression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP 150TAG
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationN150TAGPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-Acridone-RS1 (A7)
Plasmid#197566PurposeExpression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cellsDepositorInsertsM. alvus PylRS - Acd RS1 (A7)
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsNoneExpressionMammalianMutationN166A, V168G, W239CPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only